Positions:

TEMPLE UNIVERSITY, COLLEGE OF ENGINEERING, Philadelphia, PA 19122

January 2012 – Current: Professor, Dept. of Bioengineering, Temple University, Philadelphia, PA

July 2009- Dec. 2011: Research Professor, Dept. of Mechanical Engineering

EXPONENT, Philadelphia, PA

December 2007-June 2009: Managing Scientist

DREXEL UNIVERSITY, Philadelphia, PA,

2008 –2010: Visiting Research Associate Professor, School of Biomedical Engineering,

HOSPITAL FOR SPECIAL SURGERY, RESEARCH DIVISION, New York, NY

December 2007 – Current: Adjunct Scientist

2006 – 2007: Director, NIAMS-funded Musculoskeletal Imaging Core Center

2003 – 2007: Director, Musculoskeletal Imaging and Spectroscopy Lab

1999 – 2007: Associate Scientist

1994 -1999: Assistant Scientist

WEILL GRADUATE SCHOOL OF MEDICAL SCIENCES, CORNELL UNIVERSITY, New York, NY

1/2004 – December 2007: Associate Professor, Program in Physiology, Biophysics, and Systems

Biology

THE CITY UNIVERSITY OF NEW YORK, New York, NY

2002 – December 2007: Associate Professor of Biomedical Engineering, Dept. of Mechanical

Engineering

1999 – 2002: Visiting Associate Professor of Biomedical Engineering, Dept. of Mechanical

Engineering

1994 -1998: Visiting Assistant Professor of Biomedical Engineering, Dept. of Mechanical

Engineering

Research Interests: Molecular imaging of tissues and cells; Application and development of

spectroscopic analysis techniques to cartilage and bone pathologies; Tissue engineering;

Contribution of molecular alterations in connective tissues to mechanical behavior;

Characterization of biomaterials for tissue engineering and repair; MicroCT and magnetic

resonance imaging of connective tissues.

Education:

HOSPITAL FOR SPECIAL SURGERY, New York, NY

Nancy Pleshko – October 2012

2

1992 – 1994: NIH T32 Postdoctoral Fellow, Biomechanics and Ultrastructural Biochemistry

Advisors: Adele Boskey, Ph.D. and Clare Rimnac, Ph.D.

RUTGERS UNIVERSITY, Piscataway, NJ

1991 – 92: Postdoctoral Research Associate, Protein Crystallography, Dept. of Chemistry,

Advisor: Helen Berman, Ph.D.

RUTGERS UNIVERSITY, Dept. of Chemistry, Newark, NJ

1988 – 1991: Ph.D. Chemistry 1991

Thesis: “Applications of Fourier Transform Infrared Microscopy to Biomineralization”.

Thesis advisors: Richard Mendelsohn, Ph.D. and Adele Boskey, Ph.D.

RUTGERS UNIVERSITY, Dept. of Chemistry, Newark, NJ

1986 – 1988: M.S. Medicinal Chemistry, 1988

MCGILL UNIVERSITY, Montreal, Quebec, Canada

1981 -1985: B.Sc. Chemistry 1985, with Great Distinction

Research Support:

Current

Title: Evaluation of Cartilage Tissue Engineering Strategies by IR imaging

Funding Agency: NIH/NIAMS R01 AR056145 PI: Pleshko

Project dates: 8/1/09 – 5/31/13

Annual Direct Costs: $250,000

The major goal of this project is to develop infrared imaging strategies to assess engineered

construct development in situ.

Pending

Title: Application and Evaluation of Pulsed Low-Intensity Ultrasound for Repair of Osteochondral

Defects

Funding Agency: Musculoskeletal Transplant Foundation PI: Pleshko

Project dates: 1/1/2013 – 12/31/15

Annual Direct Costs: $100,000

The major goal of this project is investigate augmentation of cartilage repair with pulsed low

intensity ultrasound (PLIUS) and to develop accurate assessment of tissue molecular

characteristics that correlate with the quality of repair tissue.

Title: Response of Imaging Biomarkers to Successful Treatment of Idiopathic OA

Funding Agency: Arthritis Foundation PI: Pleshko

Project dates: 1/1/2013 – 12/31/14

Annual Direct Costs: $100,000

The major goal of this project is develop spectroscopic imaging biomarkers to evaluate cartilage

repair and regeneration in a pre-clinical model of successful treatment of osteoarthritis.

Completed

Title: Infrared Fiber Optic Imaging of Degenerative Cartilage

Funding Agency: NIH/NIBIB R01 EB00744 PI: Pleshko

Project Dates: 08/015/02–07/31/12

Annual Direct Costs: $225,000

The major goal of this project is to develop methodology for the clinical evaluation of cartilage

degeneration and repair by infrared fiber optic probe.

Nancy Pleshko – October 2012

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Title: Efficacy of RANKL Inhibition in Adult oim/oim Mice

Funding Agency: Amgen Corp and Osteogenesis Imperfecta Foundation PI: Pleshko/Raggio

Project dates: 07/02/2010 – 06/30/2011

Annual Direct Costs: $80,000

The major goal of this project is to assess the potential benefits of treatment of adult oim/oim mice

with a RANKL inhibitor.

Title: Development of Novel mid-Infrared Fiber Optic Probe (IFOP) to Study Arterial Remodeling

after Vascular Interventions

Funding Agency: Cornell NIH CTSC PI: Pleshko

Project Dates: 12/01/07 – 05/31/09

The major goal of this project is to develop infrared spectral parameters and fiber optic probe

methodology for the clinical evaluation of vascular interventions.

Title: Direct Comparison of RANKL Blockade and Bisphosphonate Therapy in OI

Funding Agencies: Amgen Corp. (PI Pleshko), and OI Foundation (Michael Geisman Fellowship

Award for Dr. Renee Bargman)

Project Dates: 7/01/07 – 06/30/09

Annual Direct Costs: $125,000

The major goal of this project is to assess whether RANKL inhibition is as effective a treatment as

bisphosphonates in growing oim/oim mice, a model of osteogenesis imperfecta

Title: Identification of Skin Abnormalities in OI Patients by MR Imaging

Funding Agency: Pediatric Society of North America PI: Raggio

Project Dates: 1/01/08 – 12/31/08

Annual Direct Costs: $27,000

The major goal of this project is to assess the ability of MRI to differentiate between skin biopsies

of OI and non-OI patients. If successful, this project will enable the design of a novel, rapid, noninvasive

modality of diagnosis for children with osteogenesis imperfecta.

Title: Architectural Microfibrils in Bone Physiology

Funding Agency: NIH/NIAMS; PI: F. Ramirez/ Sub-contract P.I: Pleshko Camacho

Project Dates: 3/01/06-02/28/08; Annual Sub Contract Direct Costs: $75,000

The major goal of this project is to utilize infrared imaging spectroscopy to evaluate extracellular

matrix abnormalities in fibrillin mutant mice. We seek to validate the hypothesis that architectural

microfibrils play two distinct roles in bone physiology – the role of regulators of signaling molecules

to modulate bone formation, growth and remodeling, and the role of a structural support for the

matrix to impart bone strength.

Title: Core Center for Musculoskeletal Repair and Regeneration – Imaging Core

Funding Agency: NIH/NIAMS; PI: Boskey

Project Dates: 3/1/06-2/281/11; Annual Direct Costs: $400,000

The Core Center for Musculoskeletal Repair and Regeneration provides facilities and an

infrastructure in which sharing of ideas and resources enhances interactions of Investigators.

Members of the research base are investigating scientific questions related to the development

and repair of bone and cartilage in health and disease, and utilize microscopic imaging, analytical

microscopy, and biomechanics techniques as part of their investigations.

Title: Spectroscopic Identification and Removal of PMMA from Bone

Funding Agency: NIH/NIAMS R41AR052968 STTR with Remspec Corp.; PI: Pleshko Camacho

Nancy Pleshko – October 2012

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Project Dates: 09/10/05-012/31/06; Annual Direct Costs: $32,760

This grant provides Phase I funding to determine the feasibility of utilizing an infrared fiber optic

probe to identify and assist in removal of PMMA from bone during revision hip arthroplasties.

Title: Anti-Resorptives for Dysregulated Bone Remodeling in Osteogenesis Imperfecta

Funding Agency: NIH/NIAMS R01 AR48337; PI: (Pleshko) Camacho

Project Dates: 10/1/01 – 9/30/07; Annual Direct Costs: $250,000

The major goals of this project are 1. To evaluate if an osteoclastic defect contributes to the

dysregulated bone remodeling in the collagen-based disease, osteogenesis imperfect (OI); and 2.

To study the effect of an anti-resorptive, OPG, on bone properties in oim/oim mice, an animal

model of OI. Infrared imaging spectroscopy, microCT, and biomechanics was utilized to assess

matrix abnormalities in these mice.

Title: Cartilage Cell and Matrix Response to Joint Loading

Funding Agency: NIH/NIAMS R01 AR045748; PI: P. Torzilli

Project Dates: 07/01/04-06/30/09; Annual Direct Costs: $250,000

The major goal of this project is to characterize the sequence of catabolic (enzymatic) events

involved in the initiation of matrix damage by excessive mechanical load, to delineate the

mechanisms of damage to aggrecan and collagen at the molecular level, to correlate these events

and damage to the functional properties of the tissue, and to compare these parameters to those

obtained for IL-1─induced damage.

Title: Maturational Changes in Superficial Zone Chondrocytes

Funding Agency: NIH/NIAMS R03 AR049062; PI: C. Hidaka

Project Dates: 03/09/04-01/31/07; Annual Direct Costs: $50,000

Project: To investigate whether enhanced remodeling by chondrocytes may be important for

integrative repair.

Title: Evaluation of Degenerative Cartilage by an Infrared Fiber Optic Probe

Funding Agency: National Medical Technology Testbed; PI: (Pleshko) Camacho

Project Dates: 6/01 – 9/04; Annual Direct Costs: $150,000

This grant provides funds to develop methodology to be used with an infrared fiber optic probe as

a tool for evaluation of cartilage degeneration.

Title: Osteogenesis Imperfecta: Bone and Dentin Abnormalities

Funding Agency: NIH DE11803; PI: (Pleshko) Camacho

Project Dates: 02/01/97 – 01/31/02; Annual Direct Costs: $70,000

The major goal of this project is to study structural abnormalities of bone and dentin in OI.

Title: Cartilage Regeneration and Repair: Analysis by Infrared Fiber Optic Probe

Funding Agency: HSS McCarthur Cartilage Fund; PI: (Pleshko) Camacho

Project Dates: 9/01/98 – 10/31/00; Annual Direct Costs: $20,000

The major goal of this project is to develop the infrared fiber optic probe as a tool for evaluation of

cartilage degeneration and repair.

Title: Effect of Bisphosphonates on Bone Properties in the Growing OIM Mouse

Funding Agency: Children’s Brittle Bone Foundation; PI: (Pleshko) Camacho

Project Dates: 6/96 – 6/98; Annual Direct Costs: $50,000

This grant provides funds to evaluate the efficacy of the bisphosphonate alendronate in treatment

of an animal model of OI.

Nancy Pleshko – October 2012

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Patents:

“Determination of the Ultrastructure of Connective Tissue by an Infrared Fiber Optic Spectroscopic

Probe”; US Patent #09/853,298 granted 12/2004; UK Patent 0226099, granted 10/2004

“Discrimination of Host from Non-Host Material With an Infrared Fiber Optic Spectroscopic Probe”;

US Patent application 60/289,984 granted 6/2006; UK Patent 03181427 granted 6/2005

Honors and Awards:

2011: Thesis Opponent – Candidate: Jarno Rieppo; Advisor: Heikki Helminen

Faculty of Health Sciences, University of Eastern Finland, Kuopio, Finland

Thesis Title: Microscopic and Spectroscopic Analysis of Immature and Mature Articular Cartilage

2008: Thesis Opponent – Candidate: Ulrike Bocker; Advisor: Achim Kohler

Department of Chemistry, Biotechnology, and Food Science

Norwegian University of Life Sciences, As, Norway

Thesis Title: “FTIR microspectroscopy of muscle food -process-related changes in secondary

structure of myofibrillar proteins and their relationships to meat functional properties”

2002: Whitehead Fellow in Musculoskeletal Research, Hosp. for Special Surgery, NY, NY

1998: Young Investigator Award, 6th Intl. Conf. on Chem. & Biol. of Mineralized Tissues

1996: Eastern Orthopaedic Association Founder’s Award

1995: Young Investigator Award, 5th Intl. Conf. on Chem. & Biol. of Mineralized Tissues

1992-94: NIH Training Grant Recipient

1986-90: Doctoral Excellence Fellow, Dept. of Chemistry, Rutgers University, Newark, NJ

Extramural Professional Responsibilities:

Journal Reviewer, including: Journal Bone and Mineral Research, Calcified Tissue Intl, Bone,

Journal of Dental Research, Connective Tissue Res, Journal Bone and Joint Surgery, Journal

Orthopaedic Research, Applied Spectroscopy, Biomacromolecules, Osteoarthritis and Cartilage,

The Journal of Clinical Endocrinology & Metabolism, Journal Structural Biology, IEEE EMB, J

Biomechanics, J Biomedical Optics, Osteoporosis Intl., Arthritis Res Therapy, Equine Veterinary

Journal, Tissue Engineering, The Analyst, PloS one, Journal of the Mechanical Behavior of

Biomedical Materials, Medical Engineering and Physics.

Ad Hoc Study Section Reviewer

NIH Musculoskeletal Tissue Engineering June 2011, September 2011, June 2012

NIH MOSS SBIR November 2011

NIH Clinical Translational Imaging May 2011

NIH Musculoskeletal Tissue Engineering September 2010

NIH AMS K awards February 2010

Austen Bioinnovation Grants December 2009

NIH NIDCR T32 January 2007

NIH NIAMS R03 March 2006

NIH SBIR Orthopaedics March 2002, July 2002

NSF 2001

NIH Oral Biology Medicine 2000

Co-Organizer and Finance Chair, 38th Northeastern Biomedical Engineering Conference, Temple

University, March 2012

The College of New Jersey Bioengineering Advisory Board 9/2009 – Current

Scientific Advisory Board, 7,8,9th Intl Conf. Chem. & Biol. of Mineralized Tissues 2002 – 2007

Session Chair and Moderator: Orthopaedic Research Society Meeting 1998, 2002

8th Intl Conference, Chem. & Biol. of Mineralized Tissues 2004

Nancy Pleshko – October 2012

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Invited Lectures: (Topics relate primarily to imaging of bone and cartilage pathologies)

Temple University Hospital Department of Orthopaedics Grand Rounds Lecture April 2012

Eastern Analytical Society, Technical Session, Somerset, NJ November 2011

University of Eastern Finland, March 2011

Federation of Analytical Chemistry and Spectroscopy Societies 37th Annual Meeting, October 2010

University of North Carolina, School of Dentistry, October 2010

Temple University, Department of Biology, October 2010

Drexel University, Dept. of Materials Science, April 2010

National Institute on Aging, Harbor Hospital, July 2009

Orthopaedic Research Society, Imaging Workshop, Las Vegas, NV February 2009

4th Clinical Meeting on Osteogenesis Imperfecta, Baltimore, MD October 2008

Third Annual Biomotion Meeting, Lake Tahoe, October 2006

DePuy Mitek, Raynham, MA June 2006

SPEC2006 Meeting, “Optical Diagnosis for the New Millenium”, Heidelberg, Germany May 2006

American Society for Pharmacology & Experimental Therapeutics, San Francisco, April 2006

International Cartilage Repair Society Meeting, January 2006

Eastern Analytical Society, Technical Session, Somerset, NJ November 2005

SPEC2004 Meeting, “Optical Diagnosis for the New Millenium”, Newark, NJ, June 2004

Nat’l Institute of Advanced Industrial Science and Technology, Tsukuba, Japan, May 2004

Northeastern Ohio Universities School of Medicine, Rootstown, OH, May 2004

Osteogenesis Imperfecta Research Advances Seminar, New York, NY, October 2003

National Institute of Standards and Technology, Gaithersburg, MD, October 2002

Shriner’s Hospital, Joint Diseases Laboratory, Montreal, Canada, June 2002

National Institutes of Health, National Institute on Aging, Baltimore, MD, April 2002

New Jersey Bone and Cartilage Group, New Brunswick, NJ, March 2002

Osteogenesis Imperfecta Research Advances Seminar, Chicago, IL, December 2001

New York Mineralized Tissue Seminar Series, NYC, NY, November 2001

Orthopaedic Research Society Infrared Imaging Workshop, Orlando, FL, March 2000

New Jersey OI Foundation, West Orange, NJ, May 1999

American College of Rheumatology Meeting, Washington D.C., 1997

Professional Memberships and Committees:

Orthopaedic Research Society Basic Science Committee (January 2011 – Current)

Orthopaedic Research Society

International Cartilage Repair Society

Society for Applied Spectroscopy

Thesis Advisor and Postgraduate-Scholar Sponsor

Masters Students:

Angela Baechtold, University of North Carolina, Dept. of Pediatric Dentistry

Thesis Title: “Dentin Abnormalities in OIM Mice” M.S. May 2000

George Kim, City University of New York, Dept. of Biomedical Engineering

Thesis Title: “IR Imaging of Cartilage Degeneration” May 2003 – 2005

Jenny Zhu, Resident, Columbia University Dental School,

Thesis Title:”Assessing Bone Resorption by Infrared Imaging”, 5/2006 – 6/2007

Temple University Mechanical Engineering Masters Students:

Somaieh Moghadam, 9/2009- 5/2010; Alireza Hosseini, 9/2009- 5/2011

Megan Obrien, 9/2010- 5/2012t; Claire Ya-Ting Ou 11/2010 – 5/2012

Mughda Padalkar, 9/2009- 12/2010,

Thesis Title: Spectroscopic Evaluation of Water in Hyaline Cartilage

Nancy Pleshko – October 2012

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Madhuri Penmatsa, 9/2009- 12/2010

Thesis Title: Infrared Spectral Imaging Analysis of Cartilage Repair Tissue

Michael Shockley, 9/2011 – current

PhD Students:

Paul West, City University of New York, Dept. of Mech Eng, NY, NY Ph.D. 5/2002

Thesis Title: “Development of an IR Fiber Optic Probe for Evaluation of Cartilage Degeneration”

Arash Hanifi, Temple University, Mechanical Engineering, 9/2009- 8/2012

Thesis Title: “Improved Specificity of Matrix Assessment of Normal and Repair Cartilage Using

Infrared Imaging Analysis and Multivariate Methods”

Doruk Baykal, Drexel University, School of Biomedical Engineering, 3/2008- current

Uday Palukuru, Temple University, Mechanical Engineering, 1/2010- current

Rabee Cheheltani (Co-mentor with Mohammad Kiani), 1/2010 – current

Mugdha Padalkar, 1/2012 – current

Postdoctoral Fellows:

Hospital for Special Surgery:

Xu Yang MD,, 1998 – 2002; Edith McCarthy, MD, 1999 – 2002;

Guiyang Li PhD 2003 – 2005; Xiaohong Bi, PhD, 2003 – 2006;

Hao Zhang MD, 2003 – 2006; Renee Bargman, MD 3/2007- 6/2009

Temple University:

Beril Kavukuogclu PhD, 9/2009- 6/2011; Cushla McGoverin PhD, 1/2012 – Current

Teaching and Curriculum Development Activities:

Summer, Fall 2012 Course Coordinator and Developer,

Introduction to Bioengineering (Graduate level team-taught)

7/2011 – 5/2012 Course Coordinator, Bionic Human, Temple University

1/2010 – Current Lecturer, Musculoskeletal Biology – TUSM Graduate level course

9/2009 – 5/2012 Lecturer, Bionic Human -Undergraduate General Education Team-taught Course

2005 – 2007 Hospital for Special Surgery – Musculoskeletal Core Center Education Lectures

University Committee Activities and Service:

2009 – 2011 Mechanical Engineering Preliminary and Qualifying Exam Committee

2009 – Current University Marshall

Publications:

Peer Reviewed Articles:

1. Pleshko N, Mendelsohn R, Boskey A: A Novel IR Spectroscopic Method for the Determination

of Crystallinity of Hydroxyapatite Minerals. Biophys J, 60:786, 1991.

2. Pleshko N, Boskey A L, Mendelsohn R: An Infrared Study of the Interaction of Polymethyl

Methacrylate with the Protein and Mineral Components of Bone. J Histochem Cytochem, 40:1413,

1992.

3. Pleshko N, Mendelsohn R, Boskey AL: An FT-IR Investigation of the Effects of Tissue

Preservation on Bone. Calcif Tissue Int, 51:72, 1992.

4. Boskey AL, Pleshko N, Doty SB, Mendelsohn R. Applications of FT-IR Microscopy to the Study

of Mineralization in Bone and Cartilage. Cells and Materials, 2:209-221, 1992.

Nancy Pleshko – October 2012

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5. Boskey A, Pleshko N, Mendelsohn R, Doty S, Binderman I: FT-IR Microscopic Mapping of

Early Mineralization in Chick Limb-bud Mesenchymal Cell Cultures. Calcif Tissue Int, 51:443,

1992.

6. Camacho NP, Smith D, Goldman A, Schneider B, Young P, Berman H: Structure of an

Interleukin-1 Mutant with Reduced Bioactivity Shows Multiple Subtle Changes in Conformation

that Affect Protein-Protein Recognition. Biochemistry, 32:8749, 1993.

7. Camacho NP, Rimnac C, Meyer Jr RA, Doty SB, Boskey, AL: Effect of Abnormal

Mineralization on the Mechanical Behavior of X-linked Hypophosphatemic Mice Femora. Bone,

17:271-278, 1995.

8. Boskey AL, Camacho NP, Gadaleta S, Paschalis EP, Mendelsohn R: Applications of Fourier

Transform Infrared Microscopy to the Study of Biologic Mineralization. L’Eurobiologiste, 30:15-23,

1996.

9. Gadaleta S, Camacho NP, Mendelsohn R, Boskey AL: Fourier Transform Infrared Microscopy

of Mineralized Turkey Tendon. Calcif Tissue Int, 58:17-23, 1996.

10. Camacho NP, Landis WJ, Boskey AL: Mineral Changes in a Mouse Model of Osteogenesis

Imperfecta Detected By Fourier Transform Infrared Microscopy. Conn Tissue Res, 35:259-265,

1996.

11. Derfus BA, Kurtin SM, Camacho NP, Kurup I, Ryan LM. Comparison of Matrix Vesicles

Derived From Normal and Osteoarthritic Human Cartilage. Conn Tissue Res, 35: 1996.

12. Camacho NP, Paschalis EP, Fratzl P, Boskey AL: Analysis of Bone Ultrastructure by Fourier

Transform Infrared Microscopy. J Fur Mineralstoffwechsel, 3 Jahrgang 1996. Special Edition 5/96,

ISSN 1023-7763, p19-20.

13. Pienkowski D, Doers TM, Monier-Faugere MC, Geng Z, Camacho NP, Boskey AL, Malluche

HH: Calcitonin alters bone quality in beagle dogs. J Bone Miner Res, 12:1936-1943, 1997.

14. Camacho NP, Dow D, Toledano TR, Buckmeyer JK, Gertner JM, Brayton CF, Raggio CL,

Root L, Boskey AL: Identification of the oim Mutation by Dye Terminator Chemistry Combined

With Automated Direct DNA Sequencing. J Orthop Res 16:38-42, 1998.

15. Derfus B, Kranendonk S, Camacho N, Mandel N, Kushnaryov V, Lynch K, Ryan L: Human

Osteoarthritic Cartilage Matrix Vesicles Generate Both Calcium Pyrophosphate Dihydrate and

Apatite In Vitro. Calcif Tissue Int, 63(3): 258-262, 1998.

16. Baechtold A , Wright JT, Yamauchi M, Spevak M, Camacho NP Dentin Composition and

Structure in the oim Mouse. In: Proceedings, 6th Intl. Conference on the Chemistry and Biology of

Mineralized Tissues. Goldberg M, Robinson C, Boskey A, editors. Rosemont, IL: AAOS

Publications, pp.57-61, 1998.

17. Bonewald L, Boskey A, Camacho N, Kato Y. Mineralization by Osteocyte-like Cell Lines.

Proceedings of the 6th Intl Conference on the Chemistry & Biology of Mineralized Tissues, 1998.

Nancy Pleshko – October 2012

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18. Bostrom MPG, Camacho NP. Potential Role of Bone Morphogenetic Proteins in Fracture

Healing. Clinical Orthop,(supplement) 355s (October) 1998, S274-282.

19. Hsu, H.H.T., Camacho NP, and Anderson, H.C. Further characterization of ATP-initiated

calcification by matrix vesicles isolated from rachitic rat cartilage. Biochim et Biophysic Acta,

1416:320-332, 1999.

20. Camacho NP, Hou L, Toledano TR, Ilg WA, Brayton CF, Raggio CL, Root L, Boskey AL. The

Material Basis for Reduced Mechanical Properties in oim Mice Bones. J Bone Min Res, 14:264-

272, 1999.

21. Camacho NP, Rinnerthaler S, Paschalis EP, Mendelsohn R, Boskey AL, Fratzl P.

Complementary information on bone ultrastructure from scanning small angle x-ray scattering and

Fourier transform infrared microscopy. Bone, 25(3):287-93,1999.

22. Blank RD, Raggio CL, Giampietro PF, Camacho NP. A genomic approach to scoliosis

pathogenesis. Lupus, 8:1-5, 1999.

23. Hsu, H.H.T. and Camacho NP. Isolation of calcifiable vesicles from human atherosclerotic

aortas. Atherosclerosis, 153(2):337-348, 2000.

24. Camacho NP, Raggio CL, Blank RD Osteogenesis Imperfecta, Current Opinion in

Orthopedics, 11:341-346, 2000.

25. Hsu HH, Camacho NP, Sun F, Tawfik O, Aono H. Isolation of calcifiable vesicles from aortas

of rabbits fed with high cholesterol diets. Atherosclerosis, 153:337-48, 2000.

26. Camacho NP, West P, Torzilli P, Mendelsohn R. FTIR Microscopic Imaging of Collagen and

Proteoglycan in Cartilage. Biospectroscopy, 62:1-8, 2001.

27. Derfus BA, Camacho NP, Olmez U, Kushnaryov VM, Westfall PR, Ryan LM, and Rosenthal

AK. Transforming growth factor beta-1 stimulates articular chondrocyte elaboration of matrix

vesicles capable of greater calcium pyrophosphate precipitation. Osteoarthritis Cartilage, 9

(3):189-194, 2001.

28. Camacho NP, Raggio CL, Doty SB, Root L, Zraick V, Ilg WA, Toledano TR, Boskey AL. A

Controlled Study of the Effects of Alendronate in a Growing Mouse Model of Osteogenesis

Imperfecta, Calc Tiss Int, 69:94-101, 2001.

29. Camacho NP, West P, Griffith MH, Warren RF, Hidaka C. FT-IR imaging spectroscopy of

genetically modified bovine chondrocytes. Materials Science and Engineering C, 17:3-9, 2001.

30. Hsu HH, Camacho NP, Tawfik O, Sun F. Induction of calcification in rabbit aortas by high

cholesterol diets:roles of calcifiable vesicles in dystrophic calcification Atheroscl, 161:85-94. 2002.

31. Aparicio S, Doty SB, Camacho NP, Paschalis EP, Spevak L, Mendelsohn R, Boskey AL.

Optimal Methods for Processing Mineralized Tissues for Fourier Transform Infrared

Microspectroscopy, Calc Tiss Int, 70:422-49, 2002.

Nancy Pleshko – October 2012

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32. Bailey AJ, Miles CA, Sims TA, Camacho NP. The role of the alpha2 chain in the stabilization

of the type I collagen heterotrimer:Type I homotrimer in oim mouse tissues. J Mol Biol,

321(5):797-805, 2002.

33. McCarthy EA, Raggio CL, Hossack MD, Miller EA, Jain S, Boskey AL, Camacho NP.

Alendronate treatment for infants with osteogenesis imperfecta: demonstration of efficacy in a

mouse model. Pediatr Res, 52(5):660-70, 2002.

34. Camacho NP, Carroll P, Raggio CL. Fourier Transform Infrared Imaging Spectroscopy (FTIRIS)

of Mineralization in Bisphosphonate-Treated oim/oim Mice, Calc Tiss Int, 72(5):604-9,

2003.

35. Bonewald LF, Harris SE, Rosser J, Dallas MR, Dallas SL, Camacho NP, Boyan B, Boskey A.

von Kossa staining alone is not sufficient to confirm that mineralization in vitro represents bone

formation. Calcif Tissue Int, 72(5):537-47, 2003.

.

36. Sims TJ, Miles CA, Bailey AJ, Camacho NP. Properties of Collagen in OIM Mouse Tissues,

Conn Tiss Res, 44(Supp):202-205, 2003.

37. Anderson HC, Sipe JB, Hessle L, Dhamyamraju R, Atti E, Camacho NP, Millan JL. Impaired

calcification around matrix vesicles of growth plate and bone in alkaline phosphatase-deficient

mice. Am J Pathol, 164(3):841-7, 2004.

38. West PA, Bostrom MPG, Torzilli PA, Camacho NP. FT-IR Spectral Analysis of Degenerative

Cartilage: An IR Fiber Optic Probe and Imaging Study, Appl. Spectrosc, 58 [4] 376-381, 2004.

39. Garimella R, Bi X, Camacho N, Sipe JB, Anderson HC. Primary culture of rat growth plate

chondrocytes: an in vitro model of growth plate histotype, matrix vesicle biogenesis and

mineralization. Bone, 34(6):961-70, 2004.

40. West PA, Torzilli PA, Chen C, Lin P, Camacho NP. Fourier Transform Infrared Imaging

Spectroscopy (FT-IRIS) Analysis of Collagenase-Induced Cartilage Degradation. J Biomed

Optics, 2005 Jan-Feb;10(1):14015.

41. Kim M, Bi X, Horton, WE, Spencer RG, Camacho NP. Fourier Transform Infrared Imaging

Spectroscopic (FT-IRIS) Analysis of Tissue Engineered Cartilage: Histologic and Biochemical

Correlations. J Biomed Opt. 2005 10(3):031105.

42. Misof BG, Roschger P, Baldini T, Raggio CL, Zraick V, Root L, Boskey AL, Klaushofer K,

Fratzl P, Camacho NP. Differential Effects of Alendronate Treatment on Bone from Growing

Osteogenesis Imperfecta and Wildtype Mice. Bone, 2005 Jan;36(1):150-8

43. Morris MD, Draper ERC, Goodship AE, Matousek P, Towrie M, Parker AW, Camacho NP.

Picosecond time-gated Raman spectroscopy for transcutaneous evaluation of bone composition.

Proceedings SPIE Symposium on Biomedical Optics, 2005

44. Lopez Franco GE, Huang A, Pleshko Camacho N, Blank RD. Dental phenotype of the

col1a2(oim) mutation: DI is present in both homozygotes and heterozygotes. Bone, 2005

Jun;36(6):1039-46.

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45. Anderson HC, Harmey D, Camacho NP, Garimella R, Sipe JB, Bi X, Tague S, Terkeltaub R,

Millán JL. Sustained Osteomalacia of Long Bones Despite Major Improvement in Other

Hypophosphatasia-Related Mineral Deficits in TNAP/NPP1 Double Deficient Mice, Am J Pathol,

2005 Jun;166(6):1711-20.

46. Draper ERC, Morris MD, Camacho NP, Matousek P, Towrie M, Parker AW, Goodship AE, A

Novel Assessment of Bone using Time-Resolved Transcutaneous Raman Spectroscopy. J Bone

Miner Res. 2005 20(11):1968-72.

47. Bi X, Li G, Doty SB, Camacho NP. A Novel Method for Determination of Collagen Orientation

in Cartilage by Fourier Transform Infrared Imaging Spectroscopy (FT-IRIS). Osteoarthritis and

Cartilage, 2005 13(12):1050-8.

48. Li G, Thomson M, DiCarlo E, Yang X, Nestor B, Bostrom MPG, Camacho NP, A

Chemometric Analysis for Evaluation of Early-Stage Cartilage Degradation by Infrared Fiber Optic

Probe Spectroscopy. Appl Spectrosc. 2005 59(12):1527-33.

49. Garimella R, Bi X, Anderson HC, Camacho NP. Nature of phosphate substrate as a major

determinant of mineral type formed in matrix vesicle-mediated in vitro mineralization: An FTIR

imaging study. Bone. 2006 Jun;38(6):811-7.

50. Bi X, Yang X, Bostrom MPG, Pleshko Camacho N. Fourier Transform Infrared Imaging

Spectroscopy Investigations in the Pathogenesis and Repair of Cartilage. BBA-Biomem 2006

Jul;1758(7):934-41

51. Harmey D, Johnson KA, Zelken J, Camacho NP, Hoylaerts MF, Noda M, Terkeltaub R, Millan

JL. Elevated skeletal osteopontin levels contribute to the hypophosphatasia phenotype in akp2(-/-)

mice. J Bone Miner Res. 2006 Sep;21(9):1377-87.

52. Southern D, Lutz G, Bracilovic A, West P, Spevak M, Pleshko Camacho N, Doty S.

Histological and Molecular Structure Characterization of Annular Collagen after Intradiskal

Electrothermal Annuloplasty, HSS Journal, 1:49-54, 2006.

53. Spencer RG, Calton EF, Pleshko Camacho N. Statistical Comparison of Fourier Transform

Infrared Spectra. J Biomed Optics, 2006 Nov-Dec;11(6):064023.

54. Bi X, Yang X, Bostrom MPG, Bartusik D, Ramaswamy S, Fishbein KW, Spencer RG,

Camacho N. Fourier transform infrared imaging and MR microscopy studies detect

compositional and structural changes in cartilage in a rabbit model of osteoarthritis. Analytical and

Bioanalytical Chemistry, 2007; 387(5):1601−1612.

55. Lopez Franco GE, Huang A, Pleshko Camacho N, Stone DS, Blank RD. Increased

Young’s Modulus and Hardness of Col1a2oim Dentin. J Dental Res, 2006 Nov;85(11):1032-6.

56. Zhang H, Ricciardi BF, Yang X, Shi Y, Camacho NP, Bostrom MPG.

Polymethylmethacrylate Particles Stimulate Bone Resorption of Mature Osteoclasts In Vitro. Acta

Orthop, 2008 79:281-288.

57. Delos D, Yang X, Ricciardi BF, Myers ER, Bostrom MPG, Pleshko Camacho N. The Effects

of RANKL Inhibition on Fracture Healing and Bone Strength in a Mouse Model of Osteogenesis

Imperfecta. J Orthop Res, 2008 Feb;26(2):153-64.

Nancy Pleshko – October 2012

12

58. Canuto HC, Fishbein KW, Bajaj P, Pleshko Camacho N, Spencer RG. Optimal Methods for

the Preservation of Cartilage Samples in MRI and Correlative Biochemical Studies. J Mag Res,

2007 May;57(5):866-73.

59. Boskey A, Pleshko Camacho N. Infrared imaging of native and tissue-engineered bone and

cartilage. Biomaterials, 2007 May;28(15):2465-78.

60. Zhang H, Doty S, Hughes C, Dempster DW, Pleshko Camacho N, Increased Resorptive

Activity and Accompanying Morphological Alterations in Osteoclasts Derived from a Mouse Model

of Osteogenesis Imperfecta (oim/oim), J Cell Biochem, 2007 Nov 1;102(4):1011-20.

.

61. Miller E, Delos D, Baldini T, Wright TM, Pleshko Camacho N. Abnormal Mineral-Matrix

Interactions are a Significant Contributor to Fragility in oim/oim bone. Calc Tiss Int, 2007

Sep;81(3):206-14.

62. Yang X, Ricciardi BF, Hernandez-Soria A, Shi Y, Pleshko Camacho N, Bostrom MP, Callus

mineralization and maturation are delayed during fracture healing in interleukin-6 knockout mice.

Bone. 2007 Dec;41(6):928-36.

63. Cheng C, Conte E, Pleshko-Camacho N, Hidaka C. Differences in matrix accumulation and

hypertrophy in superficial and deep zone chondrocytes are controlled by bone morphogenetic

protein. Matrix Biol. 2007 Sep;26(7):541-53.

64. Millán JL, Narisawa S, Lemire I, Loisel TP, Boileau G, Leonard P, Gramatikova S, Terkeltaub

R, Camacho NP, McKee MD, Crine P, Whyte MP. Enzyme Replacement Therapy for Murine

Hypophosphatasia. J Bone Miner Res. 2008 Jun;23(6):777-87.

65. Pearson KJ, Baur JA, Lewis KN, Peshkin L, Price NL, Labinsky N, Kamara D, Minor RK,

Perez E, Jamieson HA, Zhang Y, Dunn SR, Sharma K, Pleshko N, Woollett LA, Csiszar A,

Ikeno Y, Le Couteur D, Becker KG, Navas P, Ingram DK, Wolf NS, Ungvari Z, Sinclair DA, and

de Cabo R. Resveratrol delays age-related deterioration and mimics aspects of dietary restriction

in mice on a standard diet . Cell Metab. 2008 Aug;8(2):157-68.

66. Leasure J, Mahmoodian R, Siegler S, Capaldi F, Pleshko N. Fourier Transform Infrared

Spectroscopic Assessment of Changes in Composition of Proteoglycans and Collagen in

Developing Human Fetal Tarsal Bones. IMECE2008-67862 pp. 553-559 2008

67. Kim M, Foo LF, Uggen C, Lyman S, Ryaby JT, Moynihan D, Grande DA, Potter HG,

Pleshko N. Evaluation of early osteochondral defect repair in a rabbit model utilizing Fourier

transform infrared imaging spectroscopy (FT-IRIS), magnetic resonance imaging (MRI) and

quantitative T2 mapping. Tissue Eng Part C Methods. 2010 Jun;16(3):355-64.

68. Herman, BC.; Kundi, R; Yamanouchi, D; Kent, KC; Liu, B; Pleshko, N

Molecular analysis of arterial remodeling: a novel application of infrared imaging. Imaging,

Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII. Ed Farkas, DL.; Nicolau, DV.;

Leif, RC.. Proceedings of the SPIE, Volume 7182 (2009)., pp. 71821H-71821H-12 (2009).

69. Bargman R, Huang A, Boskey AL, Raggio CL, Pleshko N. RANKL Inhibition Improves Bone

Properties in a Mouse Model of Osteogenesis Imperfecta. Connective Tiss Res, 2010

Apr;51(2):123-31.

Nancy Pleshko – October 2012

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70. Hawkins J, Cifuentes M, Pleshko NL, Ambia-Sobhan H, Shapses SA. Energy restriction is

associated with lower bone mineral density of the tibia and femur in lean but not obese female

rats. J Nutr. 2010 Jan;140(1):31-7.

71. Gurkan I, Ranganathan A, Yang X, Horton WE Jr, Todman M, Huckle J, Pleshko N, Spencer

RG. Modification of osteoarthritis in the guinea pig with pulsed low-intensity ultrasound treatment.

Osteoarthritis Cartilage. 2010 May;18(5):724-33. Epub 2010 Feb 6.

72. Raghavan M, Sahar ND, Wilson RH, Mycek MA, Pleshko N, Kohn DH, Morris MD.

Quantitative polarized Raman spectroscopy in highly turbid bone tissue. J Biomed Opt. 2010 May-

Jun;15(3):037001.

73. Baykal D, Irrechukwu O, Lin P-C, Fritton K, Spencer R, Pleshko N. Non-Destructive

Assessment of Engineered Cartilage Constructs Using Near Infrared Spectroscopy. Applied

Spectroscopy, 2010 Oct;64(10):1160-6.

74. Irrechukwu O, Lin PC, Fritton K, Doty S, Pleshko N, Spencer RG. Magnetic Resonance

Studies of Macromolecular Content in Engineered Cartilage Treated with Pulsed Low-intensity

Ultrasound. Tissue Eng Part A. 2011 Feb;17(3-4):407-15.

75. Reiter D, Irrechukwu O, Lin PC, Roque R, Longo D, Doty S, Pleshko N, Spencer RG

Mapping proteoglycan-bound water in cartilage: improved specificity of matrix assessment using

multiexponential transverse relaxation analysis. Mag Res Medicine. 2011 Feb;65(2):377-84.

76. Reiter D, Irrechukwu O, Lin PC, , Pleshko N, Spencer RG “Improved specificity of cartilage

matrix evaluation using multiexponential transverse relaxation analysis applied to

pathomimetically-degraded cartilage, NMR Biomed 2011 Dec;24(10):1286-94.

77. Arteaga-Solisa E, Sui-Arteaga S, Kim M, Schaffler M, Jepsen KJ, Pleshko N, Ramirez F.

Material and mechanical properties of bones deficient for fibrillin-1 or fibrillin-2 microfibrils. Matrix

Biol. 2011 Apr;30(3):188-94

78. Canuto HC, Fishbein KW, Huang A, Doty SB, Herbert RA, Peckham J, Pleshko N, Spencer

RG. Characterization of Skin Abnormalities in a Mouse Model of Osteogenesis Imperfecta

Using High Resolution Magnetic Resonance Imaging Spectroscopy. 2012 Jan;25(1):169-76.

79. Bargman R, Posham R, DiCarlo E, Boskey AL, Raggio CL, Pleshko N. Comparable

outcomes in fracture reduction and bone properties with RANKL inhibition and alendronate

treatment in a mouse model of osteogenesis imperfecta. Osteoporosis Intl, 2012 Mar;23(3):1141-

50. Epub 2011 Sep 8.

80. Kim M, Kraft JJ, Volk AC, Pugarelli J, Pleshko N, Dodge GR. Characterization of a

Cartilage-Like Engineered Biomass Using a Self-Aggregating Suspension Culture Model:

Molecular Composition Using FT-IRIS. J Orthop Res 2011 Dec;29(12):1881-7. doi:

10.1002/jor.21467. Epub 2011 May 31.

81. Mahmoodian R, Leasure J, Philip P, Pleshko N, Capaldi F, Siegler S. Changes in Mechanics

and Composition of Human Talar Cartilage Anlagen During Fetal Development. Osteoarthritis and

Cartilage, 2011 Oct;19(10):1199-1209. Epub 2011 Jul 29.

Nancy Pleshko – October 2012

14

82. Pleshko N, Grande DA, Myers KR. Nanotechnology in Orthopaedics. J Am Acad

Orthop Surg. 2012 Jan;20(1):60-2. PubMed PMID: 22207520.

83. Reiter DA, Irrechukwu O, Lin PC, Moghadam S, Von Thaer S, Pleshko N, Spencer RG.

Improved MR-based characterization of engineered cartilage using multiexponential T2 relaxation

and multivariate analysis. NMR Biomed. 2012 Mar;25(3):476-88.

84. Terajima M, Damle S, Penmatsa M, West P, Yang X, Bostrom MPG, Hidaka C, Yamauchi

M, Pleshko N. Temporal changes in collagen cross-links in spontaneous articular cartilage

repair. Cartilage, 2012 July;3(3)278-87. doi:10.1177/1947603512437736

85. Kim M, Erickson IE, Choudhury M, Pleshko N, Mauck RL. Transient exposure to TGF-β3

improves the functional chondrogenesis of MSC-laden hyaluronic acid hydrogels. Journal of the

Mechanical Behavior of Biomedical Materials, 2012 Jul;11:92-101. Epub 2012 Mar 24.

86. Connective tissue mineralization in Abcc6-/- mice, a model for pseudoxanthoma elasticum.

Kavukcuoglu NB, Li Q, Pleshko N, Uitto J. Matrix Biol. 2012 May;31(4):246-52. Epub 2012 Mar 6.

87. Cheheltani R, Rosano JM, Wang B, Sabri A, Pleshko N, Kiani MF. Fourier Transform Infrared

Spectroscopic Imaging of Cardiac Tissue to Detect Collagen Deposition after Myocardial

Infarction. J Biomed Opt. 2012 May;17(5):056014.

88. Hanifi A, Richardson JB, Kuiper JH, Roberts S, Pleshko N. Clinical outcome of autologous

chondrocyte implantation is correlated with infrared spectroscopic imaging-derived parameters.

Osteoarthritis Cartilage. 2012 Sep;20(9):988-96

89. Bargman R, Posham R, Boskey A, Carter E, DiCarlo E, Verdelis K, Raggio C, Pleshko N.

High and Low Dose OPG-Fc Cause Osteopetrosis-Like Changes in Infant Mice. Pediatric

Research, Epub Aug 27 2012

90. Hanifi A, Bi X, Yang X, Kavukcuoglu NB, Lin PC, DiCarlo E, Spencer RG, Bostrom MPG,

Pleshko N. Infrared Fiber Optic Probe Evaluation of Degenerative Cartilage Correlates to

Histological Grading. American Journal Sports Medicine, in press, 2012

Book Chapters:

Gadaleta S, Paschalis E, Camacho NP, Betts F, Mendelsohn R, Boskey AL: Fourier Transform

Infrared Spectroscopy of Synthetic and Biological Apatites: A Review. In: Mineral Scale Formation

and Inhibition, Plennum Publishing, p 283-297, 1995.

Mendelsohn R, Boskey AL, Pleshko Camacho N: Infrared Microscopy and Imaging of Hard and

Soft Tissues: Applications to Bone, Skin, and Cartilage . In: Spectrochemical Analysis Using

Infrared Multichannel Detectors, Blackwell Publishing, Edited by Bhargava and Levin, 2005.

Kavukcuoglu NB, Pleshko N: Infrared and Raman Microscopy and Imaging of Biomaterials. In:

Comprehensive Biomaterials, Elsevier Publishing, Edited by P. Ducheynne, 2012